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Convection Enhanced Delivery of Optogenetic Adeno-associated Viral Vector to the Cortex of Rhesus Macaque Under Guidance of Online MRI Images
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神经科学
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Convection Enhanced Delivery of Optogenetic Adeno-associated Viral Vector to the Cortex of Rhesus Macaque Under Guidance of Online MRI Images

Convection Enhanced Delivery of Optogenetic Adeno-associated Viral Vector to the Cortex of Rhesus Macaque Under Guidance of Online MRI Images

6,779 Views

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08:52 min

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May 23, 2019

DOI:

10.3791/59232-v

DOI:
10.3791/59232-v

•

08:52 min
May 23, 2019

•

1 Views
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1Departments of Bioengineering,University of Washington, 2Washington National Primate Research Center, 3Department of Electrical and Computer Engineering,University of Washington, 4Department of Physiology and Center for Integrative Neuroscience,University of California, San Francisco

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Here, we demonstrate magnetic resonance (MR)-guided convection enhanced delivery (CED) of viral vectors into the cortex as an efficient and simplified approach for achieving optogenetic expression across large cortical areas in the macaque brain.

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Cite this Article

Khateeb, K., Griggs, D. J., Sabes, P. N., Yazdan-Shahmorad, A. Convection Enhanced Delivery of Optogenetic Adeno-associated Viral Vector to the Cortex of Rhesus Macaque Under Guidance of Online MRI Images. J. Vis. Exp. (147), e59232, doi:10.3791/59232 (2019).

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