Convection Enhanced Delivery of Optogenetic Adeno-associated Viral Vector to the Cortex of Rhesus Macaque Under Guidance of Online MRI Images

Karam Khateeb; Devon J. Griggs; Philip  N. Sabes; Azadeh Yazdan-Shahmorad

doi:10.3791/59232

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Convection Enhanced Delivery of Optogenetic Adeno-associated Viral Vector to the Cortex of Rhesus Macaque Under Guidance of Online MRI Images

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Neurosciences

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Journal JoVE Neurosciences

Convection Enhanced Delivery of Optogenetic Adeno-associated Viral Vector to the Cortex of Rhesus Macaque Under Guidance of Online MRI Images

6,781 Views

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08:52 min

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May 23, 2019

DOI:

10.3791/59232-v

DOI:

10.3791/59232-v

•

08:52 min

May 23, 2019

•

1 Views

Karam Khateeb^1,2, Devon J. Griggs^2,3, Philip N. Sabes⁴, Azadeh Yazdan-Shahmorad^1,2,3,4

¹Departments of Bioengineering,University of Washington, ²Washington National Primate Research Center, ³Department of Electrical and Computer Engineering,University of Washington, ⁴Department of Physiology and Center for Integrative Neuroscience,University of California, San Francisco

Summary

Here, we demonstrate magnetic resonance (MR)-guided convection enhanced delivery (CED) of viral vectors into the cortex as an efficient and simplified approach for achieving optogenetic expression across large cortical areas in the macaque brain.

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Convection Enhanced Delivery of Optogenetic Adeno-associated Viral Vector to the Cortex of Rhesus Macaque Under Guidance of Online MRI Images

Summary

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