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Convection Enhanced Delivery of Optogenetic Adeno-associated Viral Vector to the Cortex of Rhesus Macaque Under Guidance of Online MRI Images
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JoVE Journal 神経科学
Convection Enhanced Delivery of Optogenetic Adeno-associated Viral Vector to the Cortex of Rhesus Macaque Under Guidance of Online MRI Images

Convection Enhanced Delivery of Optogenetic Adeno-associated Viral Vector to the Cortex of Rhesus Macaque Under Guidance of Online MRI Images

DOI:
10.3791/59232-v

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08:52 min

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May 23, 2019

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, , ,
1Departments of Bioengineering,University of Washington, 2Washington National Primate Research Center, 3電気・コンピュータ工学科,University of Washington, 4Department of Physiology and Center for Integrative Neuroscience,University of California, San Francisco

章

  • 00:04標題
  • 00:39MR-compatible Chamber Implantation
  • 02:05Viral Vector Delivery
  • 06:18Results: Validation of Viral Expression
  • 07:47Conclusion

概要

自動翻訳

自動翻訳

Here, we demonstrate magnetic resonance (MR)-guided convection enhanced delivery (CED) of viral vectors into the cortex as an efficient and simplified approach for achieving optogenetic expression across large cortical areas in the macaque brain.

タグ

Convection Enhanced DeliveryOptogeneticAdeno-associated Viral VectorCortexRhesus MacaqueMRICraniotomyDuraMR Compatible ChamberInjection GridSalineSterile Absorbable Gel FoamVitamin E Capsule

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