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巨噬细胞 ros 产生的流式细胞仪测量对 fcγr 交联的响应
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İmmünoloji ve Enfeksiyon
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JoVE Journal İmmünoloji ve Enfeksiyon
Flow Cytometric Measurement Of ROS Production In Macrophages In Response To FcγR Cross-linking
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巨噬细胞 ros 产生的流式细胞仪测量对 fcγr 交联的响应

15,235 Views

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13:20 min

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March 07, 2019

DOI:

10.3791/59167-v

DOI:
10.3791/59167-v

•

13:20 min
March 07, 2019

•

6 Views
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1Division of Immunity and Pathogenesis, Burnett School of Biomedical Sciences,University of Central Florida

Özet

Automatically generated

这项研究表明, 使用流式细胞仪检测活性氧 (ros) 的产生, 由于激活的 fcγr。该方法可用于评估吞噬细胞对免疫复合物、光化微生物或直接 fcγr 交联的抗菌和氧化还原信号功能的变化。

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Cite this Article

Shehat, M. G., Tigno-Aranjuez, J. Flow Cytometric Measurement Of ROS Production In Macrophages In Response To FcγR Cross-linking. J. Vis. Exp. (145), e59167, doi:10.3791/59167 (2019).

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