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Metabolische Mapping: Quantitative Enzym Cytochemistry und Histochemie die Aktivität des Dehydrogenases in Zellen und Geweben zu bestimmen
JoVE Journal
İmmünoloji ve Enfeksiyon
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JoVE Journal İmmünoloji ve Enfeksiyon
Metabolic Mapping: Quantitative Enzyme Cytochemistry and Histochemistry to Determine the Activity of Dehydrogenases in Cells and Tissues
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Metabolische Mapping: Quantitative Enzym Cytochemistry und Histochemie die Aktivität des Dehydrogenases in Zellen und Geweben zu bestimmen

11,223 Views

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08:36 min

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May 26, 2018

DOI:

10.3791/56843-v

DOI:
10.3791/56843-v

•

08:36 min
May 26, 2018

•

6 Views
, , ,
1Cancer Center Amsterdam, Department of Medical Biology, Academic Medical Center,University of Amsterdam, Amsterdam, The Netherlands

Özet

Automatically generated

Hier präsentieren wir ein Protokoll, das verwendet werden kann, zu mikroskopisch visualisieren und Aktivität des Dehydrogenases in Zellen und Geweben und die Kinetik, Funktion und subzelluläre Lokalisation zu quantifizieren.

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Molenaar, R. J., Khurshed, M., Hira, V. V., Van Noorden, C. J. Metabolic Mapping: Quantitative Enzyme Cytochemistry and Histochemistry to Determine the Activity of Dehydrogenases in Cells and Tissues. J. Vis. Exp. (135), e56843, doi:10.3791/56843 (2018).

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