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Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling
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JoVE Journal Biyokimya
Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling

Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling

11,261 Views

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12:57 min

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December 21, 2017

DOI:

10.3791/56820-v

DOI:
10.3791/56820-v

•

12:57 min
December 21, 2017

•

1 Views
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1Dermatoloji Anabilim Dalı,Boston University School of Medicine

Özet

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Translational regulation plays an important role in the control of protein abundance. Here, we describe a high-throughput method for quantitative analysis of translation in the budding yeast Saccharomyces cerevisiae.

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Ribosome ProfilingGenome-wideTranslationBudding YeastSaccharomyces CerevisiaeProtein TranslationTranslational RegulationCell LysatePoly(A) MRNASucrose GradientRNase ICycloheximide

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Beaupere, C., Chen, R. B., Pelosi, W., Labunskyy, V. M. Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling. J. Vis. Exp. (130), e56820, doi:10.3791/56820 (2017).

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