Translational regulation plays an important role in the control of protein abundance. Here, we describe a high-throughput method for quantitative analysis of translation in the budding yeast Saccharomyces cerevisiae.
Beaupere, C., Chen, R. B., Pelosi, W., Labunskyy, V. M. Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling. J. Vis. Exp. (130), e56820, doi:10.3791/56820 (2017).