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Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling
JoVE Journal
生化学
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JoVE Journal 生化学
Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling

Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling

DOI:

12:57 min

December 21, 2017

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  • 00:05標題
  • 00:52Extract Preparation
  • 02:43Footprint Extraction
  • 05:15Dephosphorylation
  • 07:07Reverse Transcription
  • 10:17Results: Ribo-seq Enables Genome-wide Quantification of Translation of Specific mRNAs Under Different Cellular Conditions
  • 11:59Conclusion

概要

自動翻訳

Translational regulation plays an important role in the control of protein abundance. Here, we describe a high-throughput method for quantitative analysis of translation in the budding yeast Saccharomyces cerevisiae.

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