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Genome-wide Quantification de la traduction dans la levure de bière par Ribosome profilage
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Biyokimya
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JoVE Journal Biyokimya
Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling
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Genome-wide Quantification de la traduction dans la levure de bière par Ribosome profilage

11,259 Views

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12:57 min

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December 21, 2017

DOI:

10.3791/56820-v

DOI:
10.3791/56820-v

•

12:57 min
December 21, 2017

•

1 Views
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1Dermatoloji Anabilim Dalı,Boston University School of Medicine

Özet

Automatically generated

Régulation traductionnelle joue un rôle important dans le contrôle de l’abondance de la protéine. Nous décrivons ici une méthode de haut débit pour l’analyse quantitative de la traduction dans la levure Saccharomyces cerevisiae.

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Ribosome ProfilingGenome-wideTranslationBudding YeastSaccharomyces CerevisiaeProtein TranslationTranslational RegulationCell LysatePoly(A) MRNASucrose GradientRNase ICycloheximide

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Cite this Article

Beaupere, C., Chen, R. B., Pelosi, W., Labunskyy, V. M. Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling. J. Vis. Exp. (130), e56820, doi:10.3791/56820 (2017).

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