Dergi
/
İmmünoloji ve Enfeksiyon
/
Visualização de HIV-1 Gag vinculação a gigante Unilamelares vesículas (GUV) Membranas
JoVE Journal
İmmünoloji ve Enfeksiyon
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal İmmünoloji ve Enfeksiyon
Visualization of HIV-1 Gag Binding to Giant Unilamellar Vesicle (GUV) Membranes

Visualização de HIV-1 Gag vinculação a gigante Unilamelares vesículas (GUV) Membranas

8,635 Views

•

08:21 min

•

July 28, 2016

DOI:

10.3791/54293-v

DOI:
10.3791/54293-v

•

08:21 min
July 28, 2016

•

4 Views
, ,
1Department of Microbiology and Immunology,University of Michigan Medical School, 2Department of Biophysics,University of Michigan

Özet

Automatically generated

We illustrate here an in vitro membrane binding assay in which interactions between HIV-1 Gag and lipid membranes are visually analyzed using YFP-tagged Gag synthesized in a wheat germ-based in vitro translation system and GUVs prepared by an electroformation technique.

Explore More Videos

HIV-1 GagGiant Unilamellar Vesicle (GUV)Membrane BindingLipid CompositionVirostructural ProteinsElectroformationITO-coated SlidesLipid Film FormationChloroformTemperature Control

Read Article

Cite this Article

Olety, B., Veatch, S. L., Ono, A. Visualization of HIV-1 Gag Binding to Giant Unilamellar Vesicle (GUV) Membranes. J. Vis. Exp. (113), e54293, doi:10.3791/54293 (2016).

Download .ris file

Copy

Share Video

.

Copy