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거대한 단일 층 소포 (GUV) 막 바인딩 HIV-1 개그의 시각화
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JoVE Journal İmmünoloji ve Enfeksiyon
Visualization of HIV-1 Gag Binding to Giant Unilamellar Vesicle (GUV) Membranes
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거대한 단일 층 소포 (GUV) 막 바인딩 HIV-1 개그의 시각화

8,635 Views

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08:21 min

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July 28, 2016

DOI:

10.3791/54293-v

DOI:
10.3791/54293-v

•

08:21 min
July 28, 2016

•

4 Views
, ,
1Department of Microbiology and Immunology,University of Michigan Medical School, 2Department of Biophysics,University of Michigan

Özet

Automatically generated

We illustrate here an in vitro membrane binding assay in which interactions between HIV-1 Gag and lipid membranes are visually analyzed using YFP-tagged Gag synthesized in a wheat germ-based in vitro translation system and GUVs prepared by an electroformation technique.

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Cite this Article

Olety, B., Veatch, S. L., Ono, A. Visualization of HIV-1 Gag Binding to Giant Unilamellar Vesicle (GUV) Membranes. J. Vis. Exp. (113), e54293, doi:10.3791/54293 (2016).

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