/
/
Isolement de leucocytes infiltrant de la peau de souris Utilisation de digestion enzymatique et séparation par gradient
Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.
Isolement de leucocytes infiltrant de la peau de souris Utilisation de digestion enzymatique et séparation par gradient
Bölümler
- 00:05Başlık
- 00:58Flank Skin Harvest and Infiltrating Leukocyte Isolation
- 04:28Results: Oxazolone (Ox) Challenge Results in Pronounced T Cell and Neutrophil Flank Skin Infiltration
- 06:17Conclusion
This protocol describes enzymatic digestion of mouse skin in nutrient-rich medium followed by gradient separation to isolate leukocytes. Cells thus derived can be used for diverse downstream applications. This is an effective, economical, and improved alternative to tissue dissociation machines and harsher trypsin and dispase-based tissue digestion protocols.
