'Dergi'
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同时双光子<em>在体内</em>突触输入的影像和突触后目标中的鼠标Retrosplenial的Cortex
JoVE Journal
Nörobilim
Author Produced
'Bu içeriği görüntülemek için JoVE aboneliği gereklidir.'  'Oturum açın veya ücretsiz deneme sürümünü başlatın.'
JoVE Journal Nörobilim
Simultaneous Two-photon In Vivo Imaging of Synaptic Inputs and Postsynaptic Targets in the Mouse Retrosplenial Cortex
DOI:

16:45 min

March 13, 2016

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'Bölümler'

  • 00:00'Başlık'
  • 01:27Surgery Preparation
  • 03:37Cranial Window Surgery
  • 08:30Virus Injection
  • 10:43Cranial Window Implantation
  • 13:15Imaging
  • 15:12'Sonuçlar'
  • 15:56Conclusions

Özet

'Otomatik Çeviri'

This video shows the craniotomy procedure that allows chronic imaging of neurons in mouse retrosplenial cortex using in vivo two photon microscopy in Thy1-GFP transgenic line. This approach is combined with injection of mCherry-expressing adeno-associated virus into dorsal hippocampus. These techniques allow long-term monitoring of experience-dependent structural plasticity in RSC.

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