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Simultaneous Two-photon In Vivo Imaging of Synaptic Inputs and Postsynaptic Targets in the Mouse Retrosplenial Cortex
JoVE Journal
神経科学
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JoVE Journal 神経科学
Simultaneous Two-photon In Vivo Imaging of Synaptic Inputs and Postsynaptic Targets in the Mouse Retrosplenial Cortex
DOI:

16:45 min

March 13, 2016

, , , ,

  • 00:00標題
  • 01:27Surgery Preparation
  • 03:37Cranial Window Surgery
  • 08:30Virus Injection
  • 10:43Cranial Window Implantation
  • 13:15Imaging
  • 15:12結果
  • 15:56Conclusions

概要

自動翻訳

This video shows the craniotomy procedure that allows chronic imaging of neurons in mouse retrosplenial cortex using in vivo two photon microscopy in Thy1-GFP transgenic line. This approach is combined with injection of mCherry-expressing adeno-associated virus into dorsal hippocampus. These techniques allow long-term monitoring of experience-dependent structural plasticity in RSC.

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