'Dergi'
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في وقت واحد ثنائي الفوتون<em> في فيفو</em> تصوير متشابك المدخلات والأهداف بعد المشبكي في اللحاء ماوس خلف الطحال
JoVE Journal
Nörobilim
Author Produced
'Bu içeriği görüntülemek için JoVE aboneliği gereklidir.'  'Oturum açın veya ücretsiz deneme sürümünü başlatın.'
JoVE Journal Nörobilim
Simultaneous Two-photon In Vivo Imaging of Synaptic Inputs and Postsynaptic Targets in the Mouse Retrosplenial Cortex
DOI:

16:45 min

March 13, 2016

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'Bölümler'

  • 00:00'Başlık'
  • 01:27Surgery Preparation
  • 03:37Cranial Window Surgery
  • 08:30Virus Injection
  • 10:43Cranial Window Implantation
  • 13:15Imaging
  • 15:12'Sonuçlar'
  • 15:56Conclusions

Özet

'Otomatik Çeviri'

This video shows the craniotomy procedure that allows chronic imaging of neurons in mouse retrosplenial cortex using in vivo two photon microscopy in Thy1-GFP transgenic line. This approach is combined with injection of mCherry-expressing adeno-associated virus into dorsal hippocampus. These techniques allow long-term monitoring of experience-dependent structural plasticity in RSC.

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