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同時2光子<em>インビボ</emマウス脳梁膨大後部皮質におけるシナプス入力とシナプス後ターゲットの>イメージング
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Simultaneous Two-photon In Vivo Imaging of Synaptic Inputs and Postsynaptic Targets in the Mouse Retrosplenial Cortex
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同時2光子<em>インビボ</emマウス脳梁膨大後部皮質におけるシナプス入力とシナプス後ターゲットの>イメージング

11,282 Views

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16:45 min

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March 13, 2016

DOI:

10.3791/53528-v

DOI:
10.3791/53528-v

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16:45 min
March 13, 2016

•

2 Views
, , , ,
1Department of Molecular and Cellular Neuroscience,Nencki Institute of Experimental Biology, 2Department of Biochemistry,Nencki Institute of Experimental Biology, 3Neurobiology Centre,Nencki Institute of Experimental Biology

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This video shows the craniotomy procedure that allows chronic imaging of neurons in mouse retrosplenial cortex using in vivo two photon microscopy in Thy1-GFP transgenic line. This approach is combined with injection of mCherry-expressing adeno-associated virus into dorsal hippocampus. These techniques allow long-term monitoring of experience-dependent structural plasticity in RSC.

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Łukasiewicz, K., Robacha, M., Bożycki, Ł., Radwanska, K., Czajkowski, R. Simultaneous Two-photon In Vivo Imaging of Synaptic Inputs and Postsynaptic Targets in the Mouse Retrosplenial Cortex. J. Vis. Exp. (109), e53528, doi:10.3791/53528 (2016).

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