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抛光和钢筋，颅骨变薄窗口，为长期的小鼠脑成像
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JoVE Journal Nörobilim
A Polished and Reinforced Thinned-skull Window for Long-term Imaging of the Mouse Brain
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抛光和钢筋，颅骨变薄窗口，为长期的小鼠脑成像

DOI:
10.3791/3742-v

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09:49 min

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March 07, 2012

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, , , ,
1'Fizik Bölümü',University of California, San Diego, 2Department of Engineering Science and Mechanics,Pennsylvania State University , 3Department of Neurosurgery,Pennsylvania State University , 4Section of Neurobiology,University of California, San Diego

'Bölümler'

  • 00:05'Başlık'
  • 01:40Surgery Preparation and Animal Mounting
  • 04:02The Generation of a Polished and Reinforced Thinned-skull Window
  • 07:14Imaging Preparation
  • 08:20Representative Results for Long-term Imaging and optical Manipulation of the Mouse Cortex
  • 09:30Conclusion

Özet

'Otomatik Çeviri'

'Otomatik Çeviri'

我们提出了一个方法，形成一个横跨毫米，是没有大脑的炎症个月稳定在小鼠颅骨的影像窗口。这种方法非常适合于使用双光子显微镜的血液流通，细胞动力学，细胞/血管结构的纵向研究。

'Etiketler'

Polished And Reinforced Thinned-skull WindowLong-term ImagingMouse BrainIn Vivo ImagingCortical FunctionOptical AccessIntracranial EnvironmentPoRTS WindowOptical ClarityBone Regrowth InhibitionLight Scattering ReductionInflammation ReductionTwo-photon Laser Scanning MicroscopyCerebral Blood FlowCellular FunctionAnesthetized PreparationsAwake PreparationsOptogeneticsBlood Flow Disruption

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