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Singolo Destino Mapping di celle in Zebrafish
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JoVE Journal Biyoloji
Single Cell Fate Mapping in Zebrafish
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Singolo Destino Mapping di celle in Zebrafish

13,236 Views

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07:53 min

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October 05, 2011

DOI:

10.3791/3172-v

DOI:
10.3791/3172-v

•

07:53 min
October 05, 2011

•

3 Views
, ,
1Division of Developmental Biology,Cincinnati Children’s Hospital Medical Center, 2Division of Hematology/Oncology,Cincinnati Children’s Hospital Medical Center

Özet

Automatically generated

Un metodo è descritto photoactivate singole cellule contenenti una proteina fluorescente in gabbia con assorbimento a due fotoni da un Ti: zaffiro oscillatore laser a femtosecondi. Per mappare il destino della cellula fotoattivati, immunoistochimica è usato. Questa tecnica può essere applicata a qualsiasi tipo di cellula.

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Cite this Article

Kohli, V., Rehn, K., Sumanas, S. Single Cell Fate Mapping in Zebrafish. J. Vis. Exp. (56), e3172, doi:10.3791/3172 (2011).

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