This content is Free Access.
Single Cell Fate Mapping in Zebrafish
13,236 Views
•
07:53 min
•
October 05, 2011
DOI:
Summary
Automatically generated
A method is described to photoactivate single cells containing a caged fluorescent protein using two-photon absorption from a Ti:Sapphire femtosecond laser oscillator. To fate map the photoactivated cell, immunohistochemistry is used. This technique can be applied to any cell type.
Explore More Videos
Single Cell Fate MappingZebrafishBiología del desarrolloFate MappingLineage TracingUndifferentiated Precursor CellsPhotoactivatable ProteinsFluorescence SpectrumUV ExcitationCell Tracing ProbesLong-term Cell TrackingCaged Fluorescein-dextranEmbryo Model SystemsUncaging Fluorescein-dextranSpatial ResolutionPhotoactivationNear-infrared Laser PulsesTitanium Sapphire Femtosecond LaserTwo-photon Confocal Microscopes
Read Article
Cite this Article
Kohli, V., Rehn, K., Sumanas, S. Single Cell Fate Mapping in Zebrafish. J. Vis. Exp. (56), e3172, doi:10.3791/3172 (2011).
Copy