Dergi
/
/
Single Cell Fate Mapping in Zebrafish
JoVE Journal
Biyoloji
This content is Free Access.
JoVE Journal Biyoloji
Single Cell Fate Mapping in Zebrafish

Single Cell Fate Mapping in Zebrafish

DOI:

07:53 min

October 05, 2011

, ,

Bölümler

  • 00:05Başlık
  • 01:25Two-photon Activation of Caged Fluorescein-dextran
  • 05:03Immunodetection of Uncaged Fluorescein-dextran
  • 06:04Photoactivation and Immunodetection of Caged Fluorescein-dextran
  • 07:11Conclusion

Özet

Otomatik Çeviri

A method is described to photoactivate single cells containing a caged fluorescent protein using two-photon absorption from a Ti:Sapphire femtosecond laser oscillator. To fate map the photoactivated cell, immunohistochemistry is used. This technique can be applied to any cell type.

İlgili Videolar

Read Article