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Single Cell Fate Mapping in Zebrafish
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生物学
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JoVE Journal 生物学
Single Cell Fate Mapping in Zebrafish

Single Cell Fate Mapping in Zebrafish

DOI:
10.3791/3172-v

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07:53 min

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October 05, 2011

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, ,
1Division of Developmental Biology,Cincinnati Children’s Hospital Medical Center, 2Division of Hematology/Oncology,Cincinnati Children’s Hospital Medical Center

章

  • 00:05標題
  • 01:25Two-photon Activation of Caged Fluorescein-dextran
  • 05:03Immunodetection of Uncaged Fluorescein-dextran
  • 06:04Photoactivation and Immunodetection of Caged Fluorescein-dextran
  • 07:11Conclusion

概要

自動翻訳

自動翻訳

A method is described to photoactivate single cells containing a caged fluorescent protein using two-photon absorption from a Ti:Sapphire femtosecond laser oscillator. To fate map the photoactivated cell, immunohistochemistry is used. This technique can be applied to any cell type.

タグ

Single Cell Fate MappingZebrafish発生生物学Fate MappingLineage TracingUndifferentiated Precursor CellsPhotoactivatable ProteinsFluorescence SpectrumUV ExcitationCell Tracing ProbesLong-term Cell TrackingCaged Fluorescein-dextranEmbryo Model SystemsUncaging Fluorescein-dextranSpatial ResolutionPhotoactivationNear-infrared Laser PulsesTitanium Sapphire Femtosecond LaserTwo-photon Confocal Microscopes

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