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Single Cell Fate Mapping in Zebrafish
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October 05, 2011
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A method is described to photoactivate single cells containing a caged fluorescent protein using two-photon absorption from a Ti:Sapphire femtosecond laser oscillator. To fate map the photoactivated cell, immunohistochemistry is used. This technique can be applied to any cell type.
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Single Cell Fate MappingZebrafishBiologia do DesenvolvimentoFate MappingLineage TracingUndifferentiated Precursor CellsPhotoactivatable ProteinsFluorescence SpectrumUV ExcitationCell Tracing ProbesLong-term Cell TrackingCaged Fluorescein-dextranEmbryo Model SystemsUncaging Fluorescein-dextranSpatial ResolutionPhotoactivationNear-infrared Laser PulsesTitanium Sapphire Femtosecond LaserTwo-photon Confocal Microscopes
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Kohli, V., Rehn, K., Sumanas, S. Single Cell Fate Mapping in Zebrafish. J. Vis. Exp. (56), e3172, doi:10.3791/3172 (2011).
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