'Dergi'
/
Ormancılık ve botanik
/
Detection of Neu1 Sialidase Activity in Regulating TOLL-like Receptor Activation
JoVE Journal
Ormancılık ve botanik
'Bu içeriği görüntülemek için JoVE aboneliği gereklidir.'  'Oturum açın veya ücretsiz deneme sürümünü başlatın.'
JoVE Journal Ormancılık ve botanik
Detection of Neu1 Sialidase Activity in Regulating TOLL-like Receptor Activation

Detection of Neu1 Sialidase Activity in Regulating TOLL-like Receptor Activation

DOI:
10.3791/2142-v

•

09:04 min

•

September 07, 2010

•

, , , , , ,
1Department of Microbiology and Immunology,Queen’s University – Kingston, Ontario

'Bölümler'

  • 00:00'Başlık'
  • 01:06Introduction
  • 01:48Resurrecting Frozen Macrophage Cells
  • 02:50Plating Cells for the Sialidase Assay
  • 04:42Assaying for Sialidase Activity
  • 06:50Sialidase Assay Results
  • 08:36Conclusion

Özet

'Otomatik Çeviri'

'Otomatik Çeviri'

The sialidase assay is a simple technical approach that will elucidate novel molecular mechanism(s) of TLR sensors of microbial infections and involvement in inflammatory diseases at the receptor level on the cell surface of live macrophages.

'Etiketler'

Neu1 Sialidase ActivityTOLL-like Receptor ActivationMammalian Toll-like ReceptorsPathogen-associated Molecular PatternsInfectious DiseasesInflammatory DiseasesAutoimmune DiseasesStructural Integrity Of Sensor ReceptorsLigand InteractionsSignaling ComponentsImmunological ProtectionDisease ModificationLigand-induced TLR ActivationNeu1 Sialidase ActivationNeurotrophin ReceptorsTrkATrkBMatrix Metalloproteinase-9 (MMP-9)Sialidase Assay

Article

Embed

'ÇALMA LİSTESİNE EKLE'

Usage Statistics

'İlgili Videolar'

Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce

Quantitative Imaging of Lineage-specific Toll-like Receptor-mediated Signaling in Monocytes and Dendritic Cells from Small Samples of Human Blood

Saliva, Salivary Gland, and Hemolymph Collection from Ixodes scapularis Ticks

Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells

Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1β in Human Monocyte-derived Dendritic Cells

Isolation of Murine Peritoneal Macrophages to Carry Out Gene Expression Analysis Upon Toll-like Receptors Stimulation

Isolation and Activation of Murine Lymphocytes

Flow Cytometric Analysis of Natural Killer Cell Lytic Activity in Human Whole Blood

Detection of Inflammasome Activation and Pyroptotic Cell Death in Murine Bone Marrow-derived Macrophages

A High-throughput Platform for the Screening of Salmonella spp./Shigella spp.

Read Article