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Immunologie und Infektion
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Detection of Neu1 Sialidase Activity in Regulating TOLL-like Receptor Activation
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Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Detection of Neu1 Sialidase Activity in Regulating TOLL-like Receptor Activation

Detection of Neu1 Sialidase Activity in Regulating TOLL-like Receptor Activation

DOI:
10.3791/2142-v

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09:04 min

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September 07, 2010

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, , , , , ,
1Department of Microbiology and Immunology,Queen’s University – Kingston, Ontario

Kapitel

  • 00:00Titel
  • 01:06Introduction
  • 01:48Resurrecting Frozen Macrophage Cells
  • 02:50Plating Cells for the Sialidase Assay
  • 04:42Assaying for Sialidase Activity
  • 06:50Sialidase Assay Results
  • 08:36Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

The sialidase assay is a simple technical approach that will elucidate novel molecular mechanism(s) of TLR sensors of microbial infections and involvement in inflammatory diseases at the receptor level on the cell surface of live macrophages.

Tags

Neu1 Sialidase ActivityTOLL-like Receptor ActivationMammalian Toll-like ReceptorsPathogen-associated Molecular PatternsInfectious DiseasesInflammatory DiseasesAutoimmune DiseasesStructural Integrity Of Sensor ReceptorsLigand InteractionsSignaling ComponentsImmunological ProtectionDisease ModificationLigand-induced TLR ActivationNeu1 Sialidase ActivationNeurotrophin ReceptorsTrkATrkBMatrix Metalloproteinase-9 (MMP-9)Sialidase Assay

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