The sialidase assay is a simple technical approach that will elucidate novel molecular mechanism(s) of TLR sensors of microbial infections and involvement in inflammatory diseases at the receptor level on the cell surface of live macrophages.
Amith, S. R., Jayanth, P., Finlay, T., Franchuk, S., Gilmour, A., Abdulkhalek, S., Szewczuk, M. R. Detection of Neu1 Sialidase Activity in Regulating TOLL-like Receptor Activation. J. Vis. Exp. (43), e2142, doi:10.3791/2142 (2010).