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How to Culture, Record and Stimulate Neuronal Networks on Micro-electrode Arrays (MEAs)
Bölümler
- 00:00Başlık
- 00:51Introduction
- 01:30Culturing Cortical Neurons on a Micro-Electrode Array (MEA)
- 05:03Recording From Neuronal Networks on the MEA
- 07:54Representative Recordings from Cultures On MEAs
- 08:44Conclusion
This protocol provides the necessary information for setting up, caring for, recording from and electrically stimulating cultures on MEAs. In vitro networks provide a means for asking physiologically relevant questions at the network and cellular levels leading to a better understanding of brain function and dysfunction.
Etiketler
CultureRecordStimulateNeuronal NetworksMicro-electrode Arrays (MEAs)NeuroscientistsUnderstandingDiseasesNeuropathological ObservationFluorescent MicroscopyGenetic LabelingIntracellular RecordingDissociated NeuronsSlice PreparationsGrowing Dissociated Neuronal CulturesAdvantagesSimplified ModelNetwork Activity ManipulationElectrical Stimulation SequencesMultiple ElectrodesObservable AreasMonolayer CellsMorphology ChangesImaging TechniquesLong-term Survival In Vitro
