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How to Culture, Record and Stimulate Neuronal Networks on Micro-electrode Arrays (MEAs)
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May 30, 2010
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This protocol provides the necessary information for setting up, caring for, recording from and electrically stimulating cultures on MEAs. In vitro networks provide a means for asking physiologically relevant questions at the network and cellular levels leading to a better understanding of brain function and dysfunction.
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CultureRecordStimulateNeuronal NetworksMicro-electrode Arrays (MEAs)NeuroscientistsUnderstandingDiseasesNeuropathological ObservationFluorescent MicroscopyGenetic LabelingIntracellular RecordingDissociated NeuronsSlice PreparationsGrowing Dissociated Neuronal CulturesAdvantagesSimplified ModelNetwork Activity ManipulationElectrical Stimulation SequencesMultiple ElectrodesObservable AreasMonolayer CellsMorphology ChangesImaging TechniquesLong-term Survival In Vitro
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Hales, C. M., Rolston, J. D., Potter, S. M. How to Culture, Record and Stimulate Neuronal Networks on Micro-electrode Arrays (MEAs). J. Vis. Exp. (39), e2056, doi:10.3791/2056 (2010).
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