This content is Free Access.
How to Culture, Record and Stimulate Neuronal Networks on Micro-electrode Arrays (MEAs)
53,907 Views
•
09:27 min
•
May 30, 2010
DOI:
Summary
Automatically generated
This protocol provides the necessary information for setting up, caring for, recording from and electrically stimulating cultures on MEAs. In vitro networks provide a means for asking physiologically relevant questions at the network and cellular levels leading to a better understanding of brain function and dysfunction.
Explore More Videos
CultureRecordStimulateNeuronal NetworksMicro-electrode Arrays (MEAs)NeuroscientistsUnderstandingDiseasesNeuropathological ObservationFluorescent MicroscopyGenetic LabelingIntracellular RecordingDissociated NeuronsSlice PreparationsGrowing Dissociated Neuronal CulturesAdvantagesSimplified ModelNetwork Activity ManipulationElectrical Stimulation SequencesMultiple ElectrodesObservable AreasMonolayer CellsMorphology ChangesImaging TechniquesLong-term Survival In Vitro
Read Article
Cite this Article
Hales, C. M., Rolston, J. D., Potter, S. M. How to Culture, Record and Stimulate Neuronal Networks on Micro-electrode Arrays (MEAs). J. Vis. Exp. (39), e2056, doi:10.3791/2056 (2010).
Copy