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How to Culture, Record and Stimulate Neuronal Networks on Micro-electrode Arrays (MEAs)
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Biologia
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JoVE Journal Biologia
How to Culture, Record and Stimulate Neuronal Networks on Micro-electrode Arrays (MEAs)

How to Culture, Record and Stimulate Neuronal Networks on Micro-electrode Arrays (MEAs)

53,650 Views

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09:27 min

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May 30, 2010

DOI:

10.3791/2056-v

DOI:
10.3791/2056-v

•

09:27 min
May 30, 2010

•

16 Views
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1Department of Neurology,Emory University School of Medicine, 2Coulter Department of Biomedical Engineering, Laboratory for Neuroengineering, Georgia Institute of Technology and Emory,University School of Medicine, 3Emory University School of Medicine

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CultureRecordStimulateNeuronal NetworksMicro-electrode Arrays (MEAs)NeuroscientistsUnderstandingDiseasesNeuropathological ObservationFluorescent MicroscopyGenetic LabelingIntracellular RecordingDissociated NeuronsSlice PreparationsGrowing Dissociated Neuronal CulturesAdvantagesSimplified ModelNetwork Activity ManipulationElectrical Stimulation SequencesMultiple ElectrodesObservable AreasMonolayer CellsMorphology ChangesImaging TechniquesLong-term Survival In Vitro

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Hales, C. M., Rolston, J. D., Potter, S. M. How to Culture, Record and Stimulate Neuronal Networks on Micro-electrode Arrays (MEAs). J. Vis. Exp. (39), e2056, doi:10.3791/2056 (2010).

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