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Recombinant Vescicle Isolation from Bacterial Culture to Release Soluble Protein

JoVE Journal
Biyoloji

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JoVE Journal Biyoloji

Recombinant Vescicle Isolation from Bacterial Culture to Release Soluble Protein

Recombinant Vescicle Isolation from Bacterial Culture to Release Soluble Protein

DOI:

10.3791/200248-v

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02:50 min

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June 30, 2023

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Bree R. Streather, Karen Baker, Tara A. Eastwood, Daniel P. Mulvihill

¹School of Biosciences,University of Kent

Etiketler

Recombinant Vesicle Bacterial Culture Soluble Protein VNP Sequence Tag Lysogeny Broth Terrific Broth IPTG Centrifugation Filtration Vesicle Concentration Sonication BL21DE3 Escherichia Coli MNeongreen SDS-PAGE

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