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Recombinant Vescicle Isolation from Bacterial Culture to Release Soluble Protein
JoVE Journal
Biologia
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JoVE Journal Biologia
Recombinant Vescicle Isolation from Bacterial Culture to Release Soluble Protein

Recombinant Vescicle Isolation from Bacterial Culture to Release Soluble Protein

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02:50 min

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June 30, 2023

DOI:

10.3791/200248-v

DOI:
10.3791/200248-v

•

02:50 min
June 30, 2023

•

7 Views
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1School of Biosciences,University of Kent

Tags

Recombinant VesicleBacterial CultureSoluble ProteinVNP Sequence TagLysogeny BrothTerrific BrothIPTGCentrifugationFiltrationVesicle ConcentrationSonicationBL21DE3 Escherichia ColiMNeongreenSDS-PAGE

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Recombinant Vescicle Isolation from Bacterial Culture to Release Soluble Protein. J. Vis. Exp. (Pending Publication), e200248, doi:10.3791/200248 (2023).

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