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Production and Imaging of Recombinant Protein-Filled Vesicles from E. coli
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Recombinant Vescicle Isolation from Bacterial Culture to Release Soluble Protein (Video) | JoVE
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生物学
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Recombinant Vescicle Isolation from Bacterial Culture to Release Soluble Protein

Recombinant Vescicle Isolation from Bacterial Culture to Release Soluble Protein (Video) | JoVE

Production and Imaging of Recombinant Protein-Filled Vesicles from E. coli

DOI:
10.3791/200248-v

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02:50 min

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June 30, 2023

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, , ,
1School of Biosciences,University of Kent

Tags

Keywords: Recombinant VesicleBacterial CultureSoluble ProteinVNP Sequence TagLysogeny BrothTerrific BrothIPTGCentrifugationFiltrationVesicle ConcentrationSonicationBL21DE3 Escherichia ColiMNeongreenSDS-PAGE

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