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Recombinant Vescicle Isolation from Bacterial Culture to Release Soluble Protein

JoVE Journal
生物学

This content is Free Access.

JoVE Journal 生物学

Recombinant Vescicle Isolation from Bacterial Culture to Release Soluble Protein

Recombinant Vescicle Isolation from Bacterial Culture to Release Soluble Protein

DOI:

10.3791/200248-v

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02:50 min

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June 30, 2023

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Bree R. Streather, Karen Baker, Tara A. Eastwood, Daniel P. Mulvihill

¹School of Biosciences,University of Kent

タグ

Recombinant Vesicle Bacterial Culture Soluble Protein VNP Sequence Tag Lysogeny Broth Terrific Broth IPTG Centrifugation Filtration Vesicle Concentration Sonication BL21DE3 Escherichia Coli MNeongreen SDS-PAGE

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