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الحمض النووي القائم على تحديد الأنواع السمكية البروتوكول
Bölümler
- 00:00Başlık
- 00:43Introduction
- 01:34Preparation of Fish Samples for DNA Extraction
- 02:22Genomic DNA Extraction
- 04:26PCR and Restriction Enzyme Digest
- 05:58Identification of Test Sample Species using the Agilent Bioanalyzer and RFLP Decoder Software
- 08:14Representative Analysis of Four Distinct Fish Species
- 08:44Conclusion
- 09:14Credits
Etiketler
DNA-basedFish Species IdentificationScreening MethodPCR AmplificationGenomic DNARestriction Fragment Length Polymorphism (RFLP) AnalysisAgilent 2100 BioanalyzerRFLP Pattern Matching SoftwareSpin Column-based ProtocolNucleic AcidsBinding BufferMicro-spin CupSilica-based Fiber MatrixPCR AmplificationPrimersRestriction EnzymesDigestion ReactionsDatabase Of Experimental RFLP Patterns
