DNA-based Fish Species Identification Protocol

Rachel Formosa; Harini Ravi; Scott Happe; Danielle Huffman; Natalia Novoradovskaya; Robert Kincaid; Steve Garrett

doi:10.3791/1871

ジャーナル

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生物学

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DNA-based Fish Species Identification Protocol

JoVE Journal
生物学

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JoVE Journal 生物学

DNA-based Fish Species Identification Protocol

28,973 Views

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09:15 min

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April 28, 2010

DOI:

10.3791/1871-v

DOI:

10.3791/1871-v

•

09:15 min

April 28, 2010

•

14 Views

Rachel Formosa¹, Harini Ravi¹, Scott Happe¹, Danielle Huffman¹, Natalia Novoradovskaya¹, Robert Kincaid¹, Steve Garrett¹

¹Agilent Technologies

概要

This publication describes how to use the Agilent Fish Species Identification System to identify the species of a fish by extracting DNA and performing PCR and RFLP analysis.

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DNA-based Fish Species Identification Screening Method PCR Amplification Genomic DNA Restriction Fragment Length Polymorphism (RFLP) Analysis Agilent 2100 Bioanalyzer RFLP Pattern Matching Software Spin Column-based Protocol Nucleic Acids Binding Buffer Micro-spin Cup Silica-based Fiber Matrix PCR Amplification Primers Restriction Enzymes Digestion Reactions Database Of Experimental RFLP Patterns

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DNA-based Fish Species Identification Protocol

概要

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