Journal
/
生物学
/
DNA-based Fish Species Identification Protocol
JoVE 杂志
生物学
This content is Free Access.
JoVE 杂志 生物学
DNA-based Fish Species Identification Protocol

DNA-based Fish Species Identification Protocol

DOI:
10.3791/1871-v

•

09:15 min

•

April 28, 2010

•

, , , , , ,
1Agilent Technologies

Chapters

  • 00:00Title
  • 00:43Introduction
  • 01:34Preparation of Fish Samples for DNA Extraction
  • 02:22Genomic DNA Extraction
  • 04:26PCR and Restriction Enzyme Digest
  • 05:58Identification of Test Sample Species using the Agilent Bioanalyzer and RFLP Decoder Software
  • 08:14Representative Analysis of Four Distinct Fish Species
  • 08:44Conclusion
  • 09:14Credits

Summary

自动翻译

自动翻译

This publication describes how to use the Agilent Fish Species Identification System to identify the species of a fish by extracting DNA and performing PCR and RFLP analysis.

Tags

DNA-basedFish Species IdentificationScreening MethodPCR AmplificationGenomic DNARestriction Fragment Length Polymorphism (RFLP) AnalysisAgilent 2100 BioanalyzerRFLP Pattern Matching SoftwareSpin Column-based ProtocolNucleic AcidsBinding BufferMicro-spin CupSilica-based Fiber MatrixPCR AmplificationPrimersRestriction EnzymesDigestion ReactionsDatabase Of Experimental RFLP Patterns

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

Non-radioactive in situ Hybridization Protocol Applicable for Norway Spruce and a Range of Plant Species

Fluorescence in situ hybridization (FISH) Protocol in Human Sperm

A Noninvasive Hair Sampling Technique to Obtain High Quality DNA from Elusive Small Mammals

Detection of Viral RNA by Fluorescence in situ Hybridization (FISH)

A Protocol for the Identification of Protein-protein Interactions Based on 15N Metabolic Labeling, Immunoprecipitation, Quantitative Mass Spectrometry and Affinity Modulation

Generation, Purification, and Characterization of Cell-invasive DISC1 Protein Species

Robust 3D DNA FISH Using Directly Labeled Probes

Assessing Species-specific Contributions To Craniofacial Development Using Quail-duck Chimeras

Generation of Marked and Markerless Mutants in Model Cyanobacterial Species

Fish Sperm Assessment Using Software and Cooling Devices

Read Article