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DNA-based Fish Species Identification Protocol
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April 28, 2010
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This publication describes how to use the Agilent Fish Species Identification System to identify the species of a fish by extracting DNA and performing PCR and RFLP analysis.
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DNA-basedFish Species IdentificationScreening MethodPCR AmplificationGenomic DNARestriction Fragment Length Polymorphism (RFLP) AnalysisAgilent 2100 BioanalyzerRFLP Pattern Matching SoftwareSpin Column-based ProtocolNucleic AcidsBinding BufferMicro-spin CupSilica-based Fiber MatrixPCR AmplificationPrimersRestriction EnzymesDigestion ReactionsDatabase Of Experimental RFLP Patterns
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Formosa, R., Ravi, H., Happe, S., Huffman, D., Novoradovskaya, N., Kincaid, R., Garrett, S. DNA-based Fish Species Identification Protocol. J. Vis. Exp. (38), e1871, doi:10.3791/1871 (2010).
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