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La preparación de cultivos primarios de células hematopoyéticas de la médula ósea murina por electroporación
JoVE Journal
Biyoloji
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JoVE Journal Biyoloji
The Preparation of Primary Hematopoietic Cell Cultures From Murine Bone Marrow for Electroporation
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La preparación de cultivos primarios de células hematopoyéticas de la médula ósea murina por electroporación

25,490 Views

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08:15 min

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January 06, 2009

DOI:

10.3791/1026-v

DOI:
10.3791/1026-v

•

08:15 min
January 06, 2009

•

7 Views
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1Gene Expression Division,Bio-Rad Laboratories, Inc

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Este procedimiento se describe cómo establecer cultivos primarios de células hematopoyéticas de la médula ósea murina y es seguida por la transfección utilizando el sistema de electroporación Gene Emisores MXCell.

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Kroeger, K., Collins, M., Ugozzoli, L. The Preparation of Primary Hematopoietic Cell Cultures From Murine Bone Marrow for Electroporation. J. Vis. Exp. (23), e1026, doi:10.3791/1026 (2009).

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