This procedure describes how to establish primary hematopoietic cell cultures from murine bone marrow and is followed by transfection using the Gene Pulser MXCell electroporation system.
Kroeger, K., Collins, M., Ugozzoli, L. The Preparation of Primary Hematopoietic Cell Cultures From Murine Bone Marrow for Electroporation. J. Vis. Exp. (23), e1026, doi:10.3791/1026 (2009).