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The Preparation of Primary Hematopoietic Cell Cultures From Murine Bone Marrow for Electroporation
JoVE Journal
Biologie
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JoVE Journal Biologie
The Preparation of Primary Hematopoietic Cell Cultures From Murine Bone Marrow for Electroporation

The Preparation of Primary Hematopoietic Cell Cultures From Murine Bone Marrow for Electroporation

DOI:

08:15 min

January 06, 2009

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Kapitel

  • 00:00Titel
  • 00:08Introduction
  • 00:43Harvesting Bone Marrow From Femurs and Tibiae
  • 02:07Establishing Cultures
  • 04:33Electroporating Cells
  • 07:13Transfection Results
  • 07:53Conclusion

Summary

Automatische Übersetzung

This procedure describes how to establish primary hematopoietic cell cultures from murine bone marrow and is followed by transfection using the Gene Pulser MXCell electroporation system.

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