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The Preparation of Primary Hematopoietic Cell Cultures From Murine Bone Marrow for Electroporation
JoVE 杂志
生物学
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JoVE 杂志 生物学
The Preparation of Primary Hematopoietic Cell Cultures From Murine Bone Marrow for Electroporation

The Preparation of Primary Hematopoietic Cell Cultures From Murine Bone Marrow for Electroporation

25,495 Views

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08:15 min

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January 06, 2009

DOI:

10.3791/1026-v

DOI:
10.3791/1026-v

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08:15 min
January 06, 2009

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7 Views
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1Gene Expression Division,Bio-Rad Laboratories, Inc

Summary

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This procedure describes how to establish primary hematopoietic cell cultures from murine bone marrow and is followed by transfection using the Gene Pulser MXCell electroporation system.

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Primary Hematopoietic Cell CulturesMurine Bone MarrowElectroporationPlasmid DNASiRNAGene Pulser MXcell Electroporation SystemGene Pulser Electroporation BufferTransfectionNucleic AcidsMammalian CellsDifficult-to-transfect CellsStem CellsVideo DemonstrationFemurTibia

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Kroeger, K., Collins, M., Ugozzoli, L. The Preparation of Primary Hematopoietic Cell Cultures From Murine Bone Marrow for Electroporation. J. Vis. Exp. (23), e1026, doi:10.3791/1026 (2009).

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