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The Preparation of Primary Hematopoietic Cell Cultures From Murine Bone Marrow for Electroporation
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The Preparation of Primary Hematopoietic Cell Cultures From Murine Bone Marrow for Electroporation

The Preparation of Primary Hematopoietic Cell Cultures From Murine Bone Marrow for Electroporation

DOI:
10.3791/1026-v

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08:15 min

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January 06, 2009

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, ,
1Gene Expression Division,Bio-Rad Laboratories, Inc

Bölümler

  • 00:00Başlık
  • 00:08Introduction
  • 00:43Harvesting Bone Marrow From Femurs and Tibiae
  • 02:07Establishing Cultures
  • 04:33Electroporating Cells
  • 07:13Transfection Results
  • 07:53Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

This procedure describes how to establish primary hematopoietic cell cultures from murine bone marrow and is followed by transfection using the Gene Pulser MXCell electroporation system.

Etiketler

Primary Hematopoietic Cell CulturesMurine Bone MarrowElectroporationPlasmid DNASiRNAGene Pulser MXcell Electroporation SystemGene Pulser Electroporation BufferTransfectionNucleic AcidsMammalian CellsDifficult-to-transfect CellsStem CellsVideo DemonstrationFemurTibia

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