In vivo imaging van transgene Leishmania parasieten in een Live Host
1Interdisciplinary Immunology Program,University of Iowa, and the VA Medical Center, 2Department of Biochemistry,University of Iowa, and the VA Medical Center, 3Department of Internal Medicine,University of Iowa, 4Department of Molecular Microbiology,Washington University School of Medicine, 5Division of Dermatology,Harbor-UCLA Medical Center, Hanley-Hardison Research Center, 6Interdisciplinary Immunology Program,Iowa City VA Medical Center, 7Departments of Internal Medicine, Microbiology and Epidemiology,University of Iowa
Summary
An in vivo imaging system is used to generate quantitative measurements of murine infection with the Trypanosomatid protozoan Leishmania. This is a non-invasive and non-lethal method for detecting parasites expressing luciferase within many tissues throughout the course of chronic Leishmania spp. infection.
Abstract
Distinct species of Leishmania, a protozoan parasite of the family Trypanosomatidae, typically cause different human disease manifestations. The most common forms of disease are visceral leishmaniasis (VL) and cutaneous leishmaniasis (CL). Mouse models of leishmaniasis are widely used, but quantification of parasite burdens during murine disease requires mice to be euthanized at various times after infection. Parasite loads are then measured either by microscopy, limiting dilution assay, or qPCR amplification of parasite DNA. The in vivo imaging system (IVIS) has an integrated software package that allows the detection of a bioluminescent signal associated with cells in living organisms. Both to minimize animal usage and to follow infection longitudinally in individuals, in vivo models for imaging Leishmania spp. causing VL or CL were established. Parasites were engineered to express luciferase, and these were introduced into mice either intradermally or intravenously. Quantitative measurements of the luciferase driving bioluminescence of the transgenic Leishmania parasites within the mouse were made using IVIS. Individual mice can be imaged multiple times during longitudinal studies, allowing us to assess the inter-animal variation in the initial experimental parasite inocula, and to assess the multiplication of parasites in mouse tissues. Parasites are detected with high sensitivity in cutaneous locations. Although it is very likely that the signal (photons/second/parasite) is lower in deeper visceral organs than the skin, but quantitative comparisons of signals in superficial versus deep sites have not been done. It is possible that parasite numbers between body sites cannot be directly compared, although parasite loads in the same tissues can be compared between mice. Examples of one visceralizing species (L. infantum chagasi) and one species causing cutaneous leishmaniasis (L. mexicana) are shown. The IVIS procedure can be used for monitoring and analyzing small animal models of a wide variety of Leishmania species causing the different forms of human leishmaniasis.
Protocol
1. Infection of small animals with transgenic Leishmania 1. Parasite lines Transgenic Leishmania spp. parasites expressing luciferase are generated using an episomal or an integrating vector as reported.1 2 Clonal lines are preferred. Two important points are: (a) Integrated luciferase is preferred over episomal luciferase, since in theory these parasite lines should better retain the transgene in the absence of…
Discussion
The in vivo imaging system (IVIS) provides a method for whole animal imaging or in vivo imaging experimental infection models of different forms of leishmaniasis.18,16 The Leishmania spp. parasites can be engineered to express firefly luciferase at a level that is detected in vivo with the IVIS imaging technology. One of the major advantages of this method is that it allows non-invasive visualization of Leishmania spp. inside the live animal host. This method has be…
Disclosures
The authors have nothing to disclose.
Acknowledgements
This work was funded in part by a Merit Review grant from the Department of Veterans Affairs, by NIH grants AI045540, AI067874, AI076233-01 and AI080801 (MEW), and by AI29646 (SMB). The work was performed in part during funding of CT and JG by NIH T32 AI07511.
Materials
| Material Name | Type | Company | Catalogue Number | Comment |
|---|---|---|---|---|
| D-Luciferin Potassium Salt | Reagent | Caliper LifeSciences (Formerly Xenogen) | 122796 | |
| IVIS Imaging System 200 Series | Equipment | Caliper LifeSciences | Other IVIS models that can be used include: Lumina II, Lumina XR, Kinetic, and Spectrum. |
References
- Capul, A. A., Barron, T., Dobson, D. E., Turco, S. J., Beverley, S. M. Two functionally divergent UDP-Gal nucleotide sugar transporters participate in phosphoglycan synthesis in Leishmania major. J Biol Chem. 282, 14006-14017 (2007).
- LeBowitz, J. H., Coburn, C. M., McMahon-Pratt, D., Beverley, S. M. Development of a stable Leishmania expression vector and application to the study of parasite surface antigen genes. Proc Natl Acad Sci U S A. 87, 9736-9740 (1990).
- Mureev, S., Kushnir, S., Kolesnikov, A. A., Breitling, R., Alexandrov, K. Construction and analysis of Leishmania tarentolae transgenic strains free of selection markers. Mol Biochem Parasitol. 155, 71-83 (2007).
- Chakkalath, H. R. Priming of a beta-galactosidase (beta-GAL)-specific type 1 response in BALB/c mice infected with beta-GAL-transfected Leishmania major. Infect Immun. 68, 809-814 (2000).
- Sacks, D. L., Perkins, P. V. Identification of an infective stage of Leishmania promastigotes. Science. 223, 1417-1419 (1984).
- da Silva, R., Sacks, D. L. Metacyclogenesis is a major determinant of Leishmania promastigote virulence and attenuation. Infect Immun. 55, 2802-2806 (1987).
- Spath, G. F., Beverley, S. M. A lipophosphoglycan-independent method for isolation of infective Leishmania metacyclic promastigotes by density gradient centrifugation. Exp Parasitol. 99, 97-103 (2001).
- Scott, P., Caspar, P., Sher, A. Protection against Leishmania major in BALB/c mice by adoptive transfer of a T cell clone recognizing a low molecular weight antigen released by promastigotes. J Immunol. 144, 1075-1079 (1990).
- Belkaid, Y. The role of interleukin (IL)-10 in the persistence of Leishmania major in the skin after healing and the therapeutic potential of anti-IL-10 receptor antibody for sterile cure. J Exp Med. 194, 1497-1506 (2001).
- Wilson, M. E. Local suppression of IFN-gamma in hepatic granulomas correlates with tissue-specific replication of Leishmania chagasi. J Immunol. 156, 2231-2239 (1996).
- McElrath, M. J., Murray, H. W., Cohn, Z. A. The dynamics of granuloma formation in experimental visceral leishmaniasis. J Exp Med. 167, 1927-1937 (1988).
- Ato, M. Loss of dendritic cell migration and impaired resistance to Leishmania donovani infection in mice deficient in CCL19 and CCL21. J Immunol. 176, 5486-5493 (2006).
- Ahmed, S. Intradermal infection model for pathogenesis and vaccine studies of murine visceral leishmaniasis. Infect Immun. 71, 401-410 (2003).
- Kamala, T. Hock immunization: a humane alternative to mouse footpad injections. J Immunol Methods. 328, 204-214 (2007).
- Lang, T., Goyard, S., Lebastard, M., Milon, G. Bioluminescent Leishmania expressing luciferase for rapid and high throughput screening of drugs acting on amastigote-harbouring macrophages and for quantitative real-time monitoring of parasitism features in living mice. Cell Microbiol. 7, 383-392 (2005).
- Brittingham, A., Miller, M. A., Donelson, J. E., Wilson, M. E. Regulation of GP63 mRNA stability in promastigotes of virulent and attenuated Leishmania chagasi. Mol Biochem Parasitol. 112, 51-59 (2001).
- Roy, G. Episomal and stable expression of the luciferase reporter gene for quantifying Leishmania spp. infections in macrophages and in animal models. Mol Biochem Parasitol. 110, 195-206 (2000).
- Contag, C. H. Photonic detection of bacterial pathogens in living hosts. Mol Microbiol. 18, 593-603 (1995).
- Hardy, J., Margolis, J. J., Contag, C. H. Induced Biliary Excretion of Listeria monocytogenes. Infect. Immun. 74, 1819-1827 (2006).
- Lecoeur, H. Optimization of Topical Therapy for Leishmania major Localized Cutaneous Leishmaniasis Using a Reliable C57BL/6 Model. PLoS Negl Trop Dis. 1, e34-e34 (2007).
- Lang, T., Lecoeur, H., Prina, E. Imaging Leishmania development in their host cells. Trends Parasitol. 25, 464-473 (2009).
Tags
In Vivo ImagingTransgenic Leishmania ParasitesLive HostLeishmania SpeciesVisceral LeishmaniasisCutaneous LeishmaniasisMouse ModelsParasite BurdensMicroscopyLimiting Dilution AssayQPCR AmplificationIn Vivo Imaging System (IVIS)Bioluminescent SignalLuciferase ExpressionIntradermal InjectionIntravenous InjectionLongitudinal StudiesInter-animal VariationParasite InoculaMultiplication Of ParasitesHigh SensitivityCutaneous Locations
Cite This Article
Thalhofer, C. J., Graff, J. W., Love-Homan, L., Hickerson, S. M., Craft, N., Beverley, S. M., Wilson, M. E. In vivo Imaging of Transgenic Leishmania Parasites in a Live Host. J. Vis. Exp. (41), e1980, doi:10.3791/1980 (2010).