Analyzing Responses of Mouse Olfactory Sensory Neurons Using the Air-phase Electroolfactogram Recording

Part 1. The EOG recording setup

The recording apparatus consists of a recording electrode, reference electrode, air delivery tube, specimen stage, and dissecting microscope, all anchored on an air table within a Faraday cage. Micromanipulators are used for placement of the electrodes and the air delivery tube. A continuous air stream is bubbled through distilled water to add humidity before passing through the air delivery tube and over the specimen. A 60 mm culture dish filled with Sylgard to a depth of 6-8 mm is used as a mounting surface for the specimen. A well and a channel are hollowed out of the Sylgard in the mounting dish to provide a means to electrically connect the reference electrode to the specimen via modified Ringer’s solution.

The recording electrode and the reference electrode are connected to an amplifier. Signals from the amplifier are sent to a digitizer and then to a computer. Software such as Axograph or pClamp can be used to control the stimulation protocol, to record the signal, and for subsequent analysis of the responses. An oscilloscope connected after the amplifier can be convenient for real time monitoring of the electrical potential while placing the recording electrode and during EOG recordings.

Delivery of odorant stimuli is controlled by a Picospritzer, which is connected to the same computer used for signal acquisition. The air pressure at the Picospritzer is set to 10 psi. A single air tank and regulator can be used to supply air to both the air table and the Picospritzer. A second air tank and regulator is used to provide air for the humidified air stream, as this requires a lower pressure and a large amount of airflow. Just prior to delivering an odorant stimulus, the Picospritzer output is connected to an odorant bottle. The odorant bottle is then connected to the air delivery tube.

Part 2: Preparing electrodes

The recording electrode is a chlorided silver wire in a pulled glass capillary filled with modified Ringer’s solution (135 mM NaCl, 5 mM KCl, 1 mM CaCl₂, 1.5 mM MgCl₂, 10 mM HEPES, pH 7.4, filter sterilized). The reference electrode is a chlorided silver wire.

1. Install silver wire into the electrode holder. For the recording electrode, one to two centimeters of wire should protrude from the end of the electrode holder. More wire can be left for the reference electrode.
2. To add the AgCl coat to the silver wire, position the wire in 0.1 M NaCl and connect the electrode holder to the positive terminal of a 1.5-9 V DC power source. The negative terminal of the power source should be electrically connected to the 0.1 M NaCl solution. Allow the chloriding reaction to proceed for 10 minutes. To equalize any static charge between the recording and the reference electrode, briefly touch the electrodes together before installing them on the recording apparatus.
3. Pull a glass capillary using a micropipette puller. The opening at the tip of the capillary should be around 5-10 microns in diameter.
4. Use a diamond pencil to score and break off the blunt end of the capillary so it is ~2 cm longer than the silver wire. Fire-polish the cut end with the butane torch.
5. Melt 0.5% agarose in modified Ringer’s solution. Pull a small amount of molten agarose solution into the tip of the electrode using a transfer pipette.
6. Fill the pulled capillary about 1/2 of the way with modified Ringer’s solution (a syringe that has been heated and pulled to have a long thin end is useful for this purpose). Gently flick the capillary to dislodge any air bubbles. Store the filled electrode in the storage jar with a small amount of modified Ringer’s solution in the bottom until they are ready to be used. Once a tissue sample is prepared and ready for recording, install a filled capillary over the recording electrode wire.

Part 3: Preparing odorant solutions

The odorants amyl acetate and heptaldehyde evoke large responses and are thus good choices as EOG stimulants.

1. In microcentrifuge tubes, prepare a series of dilutions of odorant in dimethyl sulfoxide (DMSO). As a starting point for a dose-response curve, prepare 10-fold dilutions from 5 M to 5 x 10^-6 M. Make fresh dilutions each day.
2. Further dilute the odorants 50-fold in water by mixing 100 μL diluted stock in DMSO with 4.9 mLs water in 2 ounce bottles with silicone stoppers. Let the solutions equilibrate in the bottles for at least 30 minutes. Note that the vapor concentration of odorants in each bottle is unknown, but will vary as a function of the concentration of odorants in the liquid phase.
3. Insert two 18-gauge needles through the silicon stopper to provide input and output ports. The ports should be plugged when the bottle is not in use.

Part 4: Recording the EOG and analyzing data

1. Sacrifice a mouse by CO₂ euthanasia or anesthetic overdose followed by decapitation. Remove the skin overlying the skull and sagittally bisect the head along the midline.
2. Mount one half of the head, medial side up, on the mounting dish. Carefully remove the septum to expose the turbinates.
3. Place the dish with mounted tissue onto the recording stage. Align the stage so that the recording location on the turbinates is centered under the microscope.
4. Turn on the air tank to deliver humidified air to the turbinate surface. Position the air delivery tube so that it is approximately 10 mm away from the recording location. The flow rate is ~600 mL/min.
5. Set the amplifier to DC mode (AC amplification will induce artifacts in the EOG signal) with low-pass filter at 1 kHz, and gain at 100X.
6. Mount recording and reference electrodes on the micromanipulators.
7. Lower the reference electrode into the well on the mounting dish and cover with modified Ringer’s solution such that it is electrically connected to the tissue.
8. Carefully lower the recording electrode onto the surface of turbinate IIb or III. The electrode should barely touch the surface of the olfactory epithelium! When the electrode comes in contact with the epithelium (i.e. completes an electrical circuit) a straight baseline will appear in the oscilloscope.
9. Attach an odorant bottle to the side port on the air delivery tube.
10. On the computer, initiate a stimulation protocol. The sampling rate for data acquisition should be 2 kHz or higher. The software will trigger an odor pulse and begin recording.
11. A typical stimulation protocol may be a 100-msec duration single pulse, paired 100-msec pulses separated by a 1-sec interval, or a 10-sec sustained pulse.
12. Allow some time between protocols so the tissue is minimally adapted. One minute is sufficient for liquid concentrations of amyl acetate and heptaldehyde up to 10^-3 M; at higher concentrations allow 5 minutes. After delivering high odor concentrations (such as at the end of a dose-response curve) residual odor may remain in the tube. Wash the air tube with 95% ethanol and dry before continuing with additional tissue samples.
13. Axograph software provides tools for measuring key parameters of the EOG signal. Such parameters include the response amplitude, latency, time to peak, and time constants of termination. It may be desirable to digitally filter traces at 25 Hz before further analysis.

Representative Results

Figure 1. Parameters for EOG analysis. Several parameters of the EOG are particularly useful for comparison of responses between mice, including the response amplitude, the latency (the time between when the stimulus is administered and the response begins), rise time (the time between the start of the response and the peak), time to peak (the time from the start of the stimulation to the peak of the response), and time constant of termination (τ, determined by fitting the decay phase of the response to a single exponential equation). For comparison of kinetic parameters such as latency, rise time, and time constant of termination, it is advisable to normalize the peak amplitude of the responses prior to analysis.

Figure 2. Representative EOG signals under different stimulation protocols. (a) Examples of EOGs from a mouse in response to stimulation with increasing concentrations of amyl acetate. The black line at the top of the panel indicates the timing and duration of odorant stimulation. The concentrations in the legend are the concentrations of the liquid solution. (b) A dose-response relation averaged from five mice. Error bars are 95% confidence intervals. A decline in the peak amplitude is often observed at very high odor concentrations. (c) An example of an EOG in response to a paired-pulse stimulation. A single short pulse of odorant elicits adaptation lasting for several seconds. (d) An example of an EOG in response to a 10-sec sustained odorant stimulation. The EOG shows desensitization during continuous odorant presentation.