JOVE JOURNAL

Genetics

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Journal (Genetics)

Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations

Adam Z. Albitar¹, Wanlong Ma¹, Maher Albitar¹

¹NeoGenomics Laboratories

Journal (Genetics)

Analysis of Termination of Transcription Using BrUTP-strand-specific Transcription Run-on (TRO) Approach

Zuzer Dhoondia¹, Ricci Tarockoff¹, Nadra Alhusini¹, Scott Medler¹, Neha Agarwal¹, Athar Ansari¹

¹Department of Biological Sciences,Wayne State University

Journal (Genetics)

Genome Editing and Directed Differentiation of hPSCs for Interrogating Lineage Determinants in Human Pancreatic Development

Zhong-Dong Shi*¹, Chew-Li Soh*¹, Zengrong Zhu*¹, Danwei Huangfu¹

¹Developmental Biology Program,Sloan Kettering Institute

Journal (Genetics)

Generation of Fluorescent Protein Fusions in Candida Species

Sara Gonia¹, Judith Berman², Cheryl A. Gale¹

¹Department of Pediatrics,University of Minnesota, ²Department of Molecular Microbiology and Biotechnology,Tel Aviv University

Journal (Genetics)

Kinetics of Lagging-strand DNA Synthesis In Vitro by the Bacteriophage T7 Replication Proteins

Alfredo J. Hernandez¹, Charles C. Richardson¹

¹Department of Biological Chemistry and Molecular Pharmacology,Harvard Medical School

Journal (Genetics)

Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards

Joshua R. Porter¹, William G. Telford², Eric Batchelor¹

¹Laboratory of Pathology, Center for Cancer Research,National Cancer Institute, ²Experimental Transplantation and Immunology Branch, Center for Cancer Research,National Cancer Institute

Journal (Genetics)

Controllable Ion Channel Expression through Inducible Transient Transfection

Matan Geron*¹, Adina Hazan*¹, Avi Priel¹

¹The Institute for Drug Research (IDR), School of Pharmacy, Faculty of Medicine,The Hebrew University of Jerusalem (HUJI)

Journal (Genetics)

Detection of Copy Number Alterations Using Single Cell Sequencing

Kristin A. Knouse^1,2,3, Jie Wu⁴, Austin Hendricks⁵

¹Koch Institute for Integrative Cancer Research, Department of Biology,Massachusetts Institute of Technology, ²Howard Hughes Medical Institute, ³Division of Health Sciences and Technology,Harvard Medical School, ⁴The Barbara K. Ostrom (1978) Bioinformatics and Computing Facility in the Swanson Biotechnology Center, Koch Institute for Integrative Cancer Research,Massachusetts Institute of Technology, ⁵BioMicro Center, Department of Biology,Massachusetts Institute of Technology

Journal (Genetics)

Using a GFP-tagged TMEM184A Construct for Confirmation of Heparin Receptor Identity

Sara Lynn N. Farwell¹, Joshua B. Slee², Yaqiu Li¹, Linda J. Lowe-Krentz¹

¹Department of Biological Sciences,Lehigh University, ²Department of Natural Science,DeSales University

Journal (Genetics)

Targeted in Situ Mutagenesis of Histone Genes in Budding Yeast

Andrea A. Duina¹, Claire E. Turkal¹

¹Biology Department,Hendrix College

Journal (Genetics)

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement

Yishai Yehuda¹, Britny Blumenfeld¹, Dan Lehmann², Itamar Simon¹

¹Dept. of Microbiology and Molecular Genetics, IMRIC, Faculty of Medicine,Hebrew University of Jerusalem, ²The Core Research Facility, IMRIC, Faculty of Medicine,Hebrew University of Jerusalem

Journal (Genetics)

Single-cell Gene Expression Using Multiplex RT-qPCR to Characterize Heterogeneity of Rare Lymphoid Populations

Thibaut Perchet¹, Sylvestre Chea¹, Milena Hasan², Ana Cumano¹, Rachel Golub¹

¹Unit for Lymphopoieseis, Immunology Department, INSERM U1223, University Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur,Institut Pasteur, ²Center for Translational Science,Institut Pasteur, INSERM UMS20

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