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Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations
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Genetics
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JoVE Journal Genetics
Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations

Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations

English

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11,415 Views

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10:41 min

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March 29, 2017

DOI:

10.3791/55130-v

DOI:
10.3791/55130-v

•

10:41 min
March 29, 2017

•

4 Views
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1NeoGenomics Laboratories

Summary

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Wild-type blocking PCR followed by direct sequencing offers a highly sensitive method of detection for low frequency somatic mutations in a variety of sample types.

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Keyword Extraction:Wild-type Blocking PCRSomatic MutationsMyd88 GeneBone Marrow SamplesExon FiveL265 HotspotM13 SequenceBlocking OligonucleotideWild-type TemplateMelting TemperatureExtension TemperatureSecondary StructureSelf-dimerization

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Albitar, A. Z., Ma, W., Albitar, M. Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations. J. Vis. Exp. (121), e55130, doi:10.3791/55130 (2017).

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