JOVE JOURNAL

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Detection of Copy Number Alterations Using Single Cell Sequencing

Kristin A. Knouse^1,2,3, Jie Wu⁴, Austin Hendricks⁵

¹Koch Institute for Integrative Cancer Research, Department of Biology,Massachusetts Institute of Technology, ²Howard Hughes Medical Institute, ³Division of Health Sciences and Technology,Harvard Medical School, ⁴The Barbara K. Ostrom (1978) Bioinformatics and Computing Facility in the Swanson Biotechnology Center, Koch Institute for Integrative Cancer Research,Massachusetts Institute of Technology, ⁵BioMicro Center, Department of Biology,Massachusetts Institute of Technology

Journal (Genetics)

Using a GFP-tagged TMEM184A Construct for Confirmation of Heparin Receptor Identity

Sara Lynn N. Farwell¹, Joshua B. Slee², Yaqiu Li¹, Linda J. Lowe-Krentz¹

¹Department of Biological Sciences,Lehigh University, ²Department of Natural Science,DeSales University

Journal (Genetics)

Targeted in Situ Mutagenesis of Histone Genes in Budding Yeast

Andrea A. Duina¹, Claire E. Turkal¹

¹Biology Department,Hendrix College

Journal (Genetics)

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement

Yishai Yehuda¹, Britny Blumenfeld¹, Dan Lehmann², Itamar Simon¹

¹Dept. of Microbiology and Molecular Genetics, IMRIC, Faculty of Medicine,Hebrew University of Jerusalem, ²The Core Research Facility, IMRIC, Faculty of Medicine,Hebrew University of Jerusalem

Journal (Genetics)

Single-cell Gene Expression Using Multiplex RT-qPCR to Characterize Heterogeneity of Rare Lymphoid Populations

Thibaut Perchet¹, Sylvestre Chea¹, Milena Hasan², Ana Cumano¹, Rachel Golub¹

¹Unit for Lymphopoieseis, Immunology Department, INSERM U1223, University Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur,Institut Pasteur, ²Center for Translational Science,Institut Pasteur, INSERM UMS20

Journal (Genetics)

From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028

Anja Greule¹, Songya Zhang¹, Thomas Paululat², Andreas Bechthold¹

¹Department of Pharmaceutical Biology and Biotechnology,Albert-Ludwigs-Universität Freiburg, Germany, ²Department of Chemistry and Biology,Universität Siegen

Journal (Genetics)

Metagenomic Analysis of Silage

Richard K. Tennant¹, Christine M. Sambles¹, Georgina E. Diffey¹, Karen A. Moore¹, John Love¹

¹Biosciences,University of Exeter

Journal (Genetics)

Detection of Inter-chromosomal Stable Aberrations by Multiple Fluorescence In Situ Hybridization (mFISH) and Spectral Karyotyping (SKY) in Irradiated Mice

Rupak Pathak¹, Igor Koturbash², Martin Hauer-Jensen^1,3

¹Division of Radiation Health, Department of Pharmaceutical Sciences, College of Pharmacy,University of Arkansas for Medical Sciences, ²Department of Environmental Health, Fay W. Boozman School of Public Health,University of Arkansas for Medical Sciences, ³Surgical Service,Central Arkansas Veterans Healthcare System

Journal (Genetics)

Conjugative Mating Assays for Sequence-specific Analysis of Transfer Proteins Involved in Bacterial Conjugation

Fettah Erdogan¹, Cristina Lento¹, Ayat Yaseen¹, Roksana Nowroozi-Dayeni¹, Sasha Kheyson¹, Gerald F. Audette^1,2

¹Department of Chemistry,York University, ²The Centre for Research on Biomolecular Interactions,York University

Journal (Genetics)

Analysis of Cap-binding Proteins in Human Cells Exposed to Physiological Oxygen Conditions

Sara Timpano*¹, Gaelan Melanson*¹, Sonia L. Evagelou¹, Brianna D. Guild¹, Erin J. Specker¹, James Uniacke¹

¹Department of Molecular and Cellular Biology,University of Guelph

Journal (Genetics)

Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus

Jie Xiang^1,2, Katie Reding¹, Leslie Pick^1,2

¹Entomology Department,University of Maryland, ²Program in Molecular and Cell Biology,University of Maryland

Journal (Genetics)

Analysis of the Ambient Particulate Matter-induced Chromosomal Aberrations Using an In Vitro System

Isabelle R. Miousse¹, Igor Koturbash¹, Marie-Cécile Chalbot², Martin Hauer-Jensen³, Ilias Kavouras², Rupak Pathak³

¹Department of Occupational and Environmental Health,University of Arkansas for Medical Sciences, ²Department of Environmental Health Sciences,University of Alabama at Birmingham, ³Division of Radiation Health,University of Arkansas for Medical Sciences

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