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Maintaining Human Glioblastoma Cellular Diversity Ex vivo using Three-Dimensional Organoid Culture
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Maintaining Human Glioblastoma Cellular Diversity Ex vivo using Three-Dimensional Organoid Culture
Capítulos
- 00:04Introduction
- 01:25Making Organoid Molds
- 02:22Preparing Single‐Cell Suspension from Glioblastoma (GBM) Adherent Culture
- 03:22Making Organoids from a Single‐Cell Suspension
- 05:23Results: Generation and Maintenance of Human GBM Diversity Ex Vivo from Patient‐Derived Cell Cultures
- 06:10Conclusion
Here, we describe a method of generating glioblastoma (GBM) organoids from primary patient specimens or patient-derived cell cultures and maintaining them to maturity. These GBM organoids contain phenotypically diverse cell populations and recreate tumor microenvironments ex vivo.
