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使用脂质单层方法进行电子晶体学研究的样品制备

JoVE Journal
Bioquímica
Author Produced

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JoVE Journal Bioquímica

Sample Preparation using a Lipid Monolayer Method for Electron Crystallographic Studies

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使用脂质单层方法进行电子晶体学研究的样品制备

3,497 Views

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04:22 min

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November 20, 2021

DOI:

10.3791/63015-v

DOI:

10.3791/63015-v

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04:22 min

November 20, 2021

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9 Views

Chloe D. Truong², Dewight R. Williams, Mary Zhu, Joseph Che-Yen Wang, Po-Lin Chiu²

¹School of Molecular Sciences,Arizona State University, ²Center for Applied Structural Discovery, Biodesign Institute,Arizona State University, ³Eyring Materials Center,Arizona State University, ⁴Department of Microbiology and Immunology,The Pennsylvania State University

Tags

Lipid Monolayer Electron Crystallography 2D Crystallization Membrane Proteins Protein Binding Sample Preparation Teflon Plate Buffer Reservoir Humidity Control Carbon-coated EM Grid

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