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双电穿孔，以定时和空间分离的细胞群
JoVE Journal
Neurociência
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JoVE Journal Neurociência
Double In Utero Electroporation to Target Temporally and Spatially Separated Cell Populations
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双电穿孔，以定时和空间分离的细胞群

7,106 Views

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10:45 min

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June 14, 2020

DOI:

10.3791/61046-v

DOI:
10.3791/61046-v

•

10:45 min
June 14, 2020

•

7 Views
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1Estructura de Recerca Interdisciplinar en Biotecnología y Biomedicina (ERI BIOTECMED), Departamento de Biología Celular, Biología Funcional y Antropología Física,Universidad de Valencia

Summary

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子宫电穿孔的双倍允许瞄准空间和时间分离的细胞群。该技术可用于在正常条件下使用荧光蛋白可视化这些细胞群之间的相互作用，而且在功能实验后也可用于干扰感兴趣的基因。

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Cite this Article

Mateos-White, I., Fabra-Beser, J., de Agustín-Durán, D., Gil-Sanz, C. Double In Utero Electroporation to Target Temporally and Spatially Separated Cell Populations. J. Vis. Exp. (160), e61046, doi:10.3791/61046 (2020).

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